天天操夜夜拍丨国产精品超清白人精品av丨天天爽天天噜在线播放丨国产精品久久久久久久福利竹菊丨色综合天天天天做夜夜夜夜做丨国内自拍xxxx18丨白人と日本人の交わりビデオ丨午夜激情在线观看丨成人18视频丨久久66热人妻偷产精品丨视频一区二区三区在线观看丨操操操插插插丨久久的久久爽亚洲精品aⅴ丨91精品一区二区三区在线观看丨aaa女人18毛片水真多丨99re在线视频精品丨超碰免费在丨中文字幕人妻无码专区app丨午夜香蕉视频丨又大又粗欧美黑人aaaaa片丨久久夫妻视频丨乌克兰性欧美精品高清丨亚洲欧美福利视频丨少妇高潮久久久久久一代女皇丨97国产高清

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > LASCPC-01
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
LASCPC-01
LASCPC-01
規格:
貨期:
編號:B161409
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 LASCPC-01
商品貨號 B161409
Organism Homo sapiens, human
Tissue prostate
Product Format frozen 1.0 mL
Morphology aggregate
Culture Properties suspension/aggregate
Biosafety Level 2  [Cells contain lentivirus genes]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease prostate cancer
Age 75-80
Gender male
Ethnicity Caucasian
Applications This cell line with the N-Myc as a driver of NEPC can serve for studying therapeutic treatments of Neuroendocrine prostate cancer (NEPC)
Karyotype Conventional karyotyping: normal 46 X,Y
Array CGH: mosaic gian of 1q23.1-1q44
Images Cell Micrograph of LASCPC-01 cells, ATCC CRL-3356
Clinical Data 75-80 years
male
Caucasian
Comments Expression of RFP, GFP, MYCN, and myristoylated AKT1.
Complete Growth Medium

To prepare 500mL of complete medium:

  • 464 mL Modified HITES medium (prepared as below)
  • 1 mL Hydrocortisone (Sigma cat# H-0135; 10 nM or 1.0 x 10-8 M)
  • 5 mL Transferrin (Sigma cat# T-5391; 0.01 mg/mL or 10 µg/mL)
  • 25 mL FBS (ATCC 30-2020)
  • 5 mL L-Glutamine (ATCC 30-2214)
Modified HITES Medium
  • Insulin, human recombinant (Life Technologies cat# 12585-014), 50 mg 
  • Sodium Selenite (Sigma cat# S9133), 0.04326 mg 
  • Β-Estradiol (Sigma cat# E2257), 0.02724 mg 
  • L-Glutamine (ATCC 30-2214), 2.922 g 
  • RPMI-1640 (ATCC 30-2001), 10 L
Adjust pH to 7.2 – 7.4. Filter sterilize through a 0.2 micrometer filter, dispense 500 mL into 500 mL bottles.

Note: Complete culture medium is stable for 1 month once hydrocortisone and transferrin are added.
Subculturing Cultures can be maintained by addition of fresh medium once or twice a week.
Cryopreservation liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial Since being aggregates we use 20 ml culture medium with healthy aggregates per 1 ml freeze volume
Volume 1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 8,10
THO1: 9
TPOX: 8,11
vWA: 15,17
Sterility Tests Bacteria and yeast: No growth 
Mycoplasma: No growth
Viability ≥ 50%
Population Doubling Time Approximately 24 hours per depositor
Name of Depositor J Lee
Year of Origin 2014
References

Lee JK, et al. N-Myc drives neuroendocrine prostate cancer initiated from human prostate epithelial cells. Canc Cell 29(4):536-47, 2016. PubMed: 27050099

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479