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EL4-Luc2

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編號:B161693

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產品名稱	EL4-Luc2
商品貨號	B161693
Organism	Mus musculus, mouse
Tissue	blood
Product Format	frozen 1.0 mL
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	lymphoma
Strain	C57BL/6N
Applications	Excellent signal/background ratio and stable luciferase expression make this cell line ideal for in vivo bioluminescence imaging of xenograft animal model to study human cancer and monitor activity of anti-cancer drugs. It also can be used in cell-based assays for cancer research.
Comments	This luciferase expressing cell line was derived from parental line (ATCC TIB-39) by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter. This cell line was established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and in vivo bioluminescence assays. The cells should be maintained in blasticidin (4 μg/mL) containing medium in routine cell culture. It is recommended to remove blasticidin prior to and during the experiment procedure when the cells are injected into animals in vivo, or co-cultured with other cell types in vitro.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Modified Eagle's Medium (DMEM, ATCC 30-2001). To make the complete growth medium, add the following components to the base medium: Horse serum (FBS; ATCC 30-2040) to a final concentration of 10% Blasticidin to a final concentration of 4 μg/mL
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵ to 4.0 x 10 ⁵ cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL. Medium Renewal: every 2 to 3 days (depending on cell density).
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Cells per Vial	≥ 1.0 x 10⁶ cells
Volume	1.0 mL
Sterility Tests	Bacteria and yeast: No growth Mycoplasma: No growth
Functional Tests	Luciferase activity: signal to noise ≥ 1,000 RLUs In Vitro Luminesence: 20,000 photons/cell/sec, subject to imaging and culturing conditions
Population Doubling Time	approximately 19 hrs
Name of Depositor	ATCC
Year of Origin	2018
References	Zinn KR, et al. Noninvasive bioluminescence imaging in small animals. ILARJ 49: 103-115, 2008. PubMed: 18172337 Dothager RS, et al. Advances in bioluminescence imaging of live animal models. Curr Opin Biotechnol 20: 45-53, 2009. PubMed: 19233638 Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252 Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344 Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916 Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019

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