| 產(chǎn)品名稱(chēng) |
XHJ-12.4 |
| 商品貨號(hào) |
B161997 |
| Designations |
XHJ-12.4 |
| GenBank Number |
J04509
|
| Species |
Mus musculus, mouse |
| Depositors |
D Nathans |
| Vector |
Construct size (kb): 4.400000095367432 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.669999957084656 Tissue: activated 3T3 cell line Gene product: Jun-D oncogene( Jun protooncogene related gene d1, Jun-D protooncogene) [Jund] |
| Insert Size (kb) |
1.670 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7; HindIII--4.7; BamHI--4.7; PstI--4.7; SacI--4.7; AccI--3.6, 1.0. jun-D appears to be expressed at a higher constitutive level than c-jun or jun-B, and is activated only slightly by serum growth factors. The insert contains the following unique sites (in bp from the T7 promoter side [5']): BstEII--446; SfiI--572; AccI--654; FspI--916; HincII--1083; DraI--1487. Although there is no characteristic poly(A) signal, there is an AGTAAA sequence near the 3' end. There is also a single ATTTA element, multiple copies of which are associated with mRNA instability. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Ryder K, et al. Jun-D: A third member of the Jun gene family. Proc. Natl. Acad. Sci. USA 86: 1500-1503, 1989. PubMed: 2493644
Howard TA, et al. A linkage map of mouse chromosome 8: further definition of homologous linkage relationships between mouse chromosome 8 and human chromosomes 8, 16, and 19. Genomics 10: 207-213, 1991. PubMed: 1675193
Daniel Nathans, personal communication
|
