ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt positions according to the published sequence): HindIII--177; EcoRV--344; PvuII--554, 1070; PstI--680, 1085; AvaI--1168; BglI--1332.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7; HindIII--3.2, 1.5; AvaI--4.1, 0.6; PstI--3.5, 1.1; BglI--2.9, 1.8.
Detects a single EcoRI fragment of 10.8 kb and a single BamHI fragment of 16.5 kb in Southern blots of genomic DNA from BC3H-1 cells.
Contains the complete coding sequence (nt 12-1571).
Detects a mRNA of 2.0 kb.
Oriented such that transcription from the SP6 promoter gives the sense strand.
