| 產(chǎn)品名稱 |
J27-neo |
| 商品貨號(hào) |
B162355 |
| Organism |
Mus musculus, mouse |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
C3H |
| Applications |
J27-neo is a stable transfected cell line established in 1987 by transfection by calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. J27-neo does not secrete or express HLA B7. |
| Derivation |
J27-neo is a stable transfected cell line established in 1987 by transfection by calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. The cells were selected in HAT and G418. |
| Comments |
J27-neo is a stable transfected cell line established in 1987 by transfection by calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. The cells were selected in HAT and G418. The vector did not carry an insert. J27-neo does not secrete or express HLA B7. J27.2 is a C3H mouse fibroblast L cell line stably expressing the tk and human b2m genomic genes. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
F Grumet |
| Year of Origin |
1987 |
| References |
Kavathas P, Herzenberg LA. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting. Proc. Natl. Acad. Sci. USA 80: 524-528, 1983. PubMed: 6188154
Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968
Cohen N, et al. Secretion of genetically engineered human/mouse class I antigens. Hum. Immunol. 25: 207-222, 1989. PubMed: 2670852
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