| 產(chǎn)品名稱 | AR42J |
|---|---|
| 商品貨號 | B163953 |
| Organism | Rattus norvegicus, rat |
| Tissue | pancreas/exocrine |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | tumor |
| Strain | Wistar |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Receptor Expression | insulin, expressed glucocorticoid, expressed |
| Genes Expressed | amylase and other exocrine enzymes Ref   Jessop NW, Hay RJ. Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors. In Vitro 16: 212, 1980. |
| Cellular Products | amylase and other exocrine enzymes Ref Jessop NW, Hay RJ. Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors. In Vitro 16: 212, 1980. |
| Tumorigenic | Yes |
| Effects | Yes, in athymic mice |
| Comments | Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20% .
|
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Monolayer never becomes confluent. Subculture when patches of cells start forming "domes".
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days. May need to only add media initially, do not fluid change until cells attach well.
Note: The cells grow slowly, in clusters. They tend to pile up and appear refractile. |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with an additional 30% (v/v) fetal bovine serum and 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor | NW Jessop |
| Deposited As | Rattus sp. |
| References | Jessop NW, Hay RJ. Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors. In Vitro 16: 212, 1980. Longnecker DS, et al. Transplantation of azaserine-induced carcinomas of pancreas in rats. Cancer Lett. 7: 197-202, 1979. PubMed: 509403 Cockell M, et al. Identification of a cell-specific DNA-binding activity that interacts with a transcriptional activator of genes expressed in the acinar pancreas. Mol. Cell. Biol. 9: 2464-2476, 1989. PubMed: 2788241 Roux E, et al. The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA. Genes Dev. 3: 1613-1624, 1989. PubMed: 2612907 Seva C, et al. Lorglumide and loxiglumide inhibit gastrin-stimulated DNA synthesis in a rat tumoral acinar pancreatic cell line (AR42J). Cancer Res. 50: 5829-5833, 1990. PubMed: 2393852 Rajasekaran AK, et al. Structural reorganization of the rough endoplasmic reticulum without size expansion accounts for dexamethasone-induced secretory activity in AR42J cells. J. Cell Sci. 105: 333-345, 1993. PubMed: 7691838 Longnecker DS, et al. Effect of age on nodule induction by azaserine and DNA synthesis in rat pancreas. J. Natl. Cancer Inst. 58: 1769-1775, 1977. PubMed: 864754 Huang Y, Hui DY. Cholesterol esterase biosynthesis in rat pancreatic AR42J cells. Post- transcriptional activation by gastric hormones. J. Biol. Chem. 266: 6720-6725, 1991. PubMed: 2016288 Menniti FS, et al. Turnover of inositol polyphosphate pyrophosphates in pancreatoma cells. J. Biol. Chem. 268: 3850-3856, 1993. PubMed: 8382679 Logsdon CD, et al. Glucocorticoids increase amylase mRNA levels, secretory organelles, and secretion in pancreatic acinar AR42J cells. J. Cell Biol. 100: 1200-1208, 1985. PubMed: 2579957 Zhao H, et al. Regulation of intracellular Ca2+ oscillation in AR42J cells. J. Biol. Chem. 265: 20856-20862, 1990. PubMed: 1701171 Zhao H, Muallem S. Inhibition of inositol 1,4,5-trisphosphate-mediated Ca2+ release by Ca2+ in cells from peripheral tissues. J. Biol. Chem. 265: 21419-21422, 1990. PubMed: 2174872 Ihara H, Nakanishi S. Selective inhibition of expression of the substance P receptor mRNA in pancreatic acinar AR42J cells by glucocorticoids. J. Biol. Chem. 265: 22441-22445, 1990. PubMed: 1702421 Adell T, et al. Role of the basic helix-loop-helix transcription factor p48 in the differentiation phenotype of exocrine pancreas cancer cells. Cell Growth Differ. 11: 137-147, 2000. PubMed: 10768861 Seva C, et al. Growth-promoting effects of glycine-extended progastrin. Science 265: 410-412, 1994. PubMed: 8023165 Negre F, et al. Autocrine stimulation of AR4-2J rat pancreatic tumor cell growth by glycine-extended gastrin. Int. J. Cancer 66: 653-658, 1996. PubMed: 8647628 Bertrand V, et al. Inhibition of gastrin-induced proliferation of AR4-2J cells by calcium channel antagonists. Int. J. Cancer 56: 427-432, 1994. PubMed: 7508895 Mashima H, et al. Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells. J. Clin. Invest. 97: 1647-1654, 1996. PubMed: 8601630 Palgi J, et al. Transcription factor expression and hormone production in pancreatic AR42J cells. Mol. Cell. Endocrinol. 165: 41-49, 2000. PubMed: 10940482 Mashima H, et al. Formation of insulin-producing cells from pancreatic acinar AR42J cells by hepatocyte growth factor. Endocrinology 137: 3969-3976, 1993. PubMed: 8756573 Silver K, Yao F. ARIP cells as a model for pancreatic beta cell growth and development. Pancreas 22: 141-147, 2001. PubMed: 11249068 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
