| 產品名稱 |
pHGH107 |
| 商品貨號 |
B197716 |
| Designations |
pHGH107 |
| Species |
Homo sapiens, human |
| Depositors |
Genentech, Inc. |
| Applications |
produces protein growth hormone 1 growth hormone GH1 |
| Vector |
Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGH6
Intact vector size: 4.600
Type of vector: plasmid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: EcoRI
Polylinker sites:
Construction: pBR322, pKB268
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
promoter: lacUV5
replicon: pMB1
Cross references: DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGH6
Intact vector size: 4.600
Type of vector: plasmid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: EcoRI
Polylinker sites:
Construction: pBR322, pKB268
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
promoter: lacUV5
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: GH1
Genomic copy number: unique
Gene name: growth hormone 1
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 q22-q24
Type of DNA: cDNA
Insert end: EcoRI
Insert end: SmaI
Insert size (kb): 0.591
Cross references: DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: GH1
Genomic copy number: unique
Gene name: growth hormone 1
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 q22-q24
Type of DNA: cDNA
Insert end: EcoRI
Insert end: SmaI
Insert size (kb): 0.591
Cross references: Insert lengths(kb): 0.5910000205039978 Gene product: growth hormone 1(growth hormone GH1) [GH1] Target Gene: growth hormone 1 |
| Insert Size (kb) |
0.591 |
| Media |
ATCC® Medium 1083: LB medium for chi 1776
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.6. For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). The insert contains sequences encoding all 191 amino acids of human growth hormone. Contains an internal PstI, BglII and PvuII site. The vector (ATCC 31539, 40012) is pBR322 modified by the insertion of 2 lac UV5 promoters at the EcoRI site. These promoters regulate expression of the insert. |
| References |
Goeddel DV, Heyneker HL. Method of constructing a replicable cloning vehicle having quasi-synthetic genes. US Patent 4,342,832 dated Aug 3 1982
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
