Gene product: P glycoprotein 1/multiple drug resistance 1 [MDR1]
Insert Size (kb)
4.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
Purified plasmid DNA: ATCC 65705
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--4.15, 3.15; EcoRI--7.3; HindIII--7.3; BglII--6.3; 1.0; PstI--7.3.
According the sequence of GenBank accession M14758, the insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--1.27; HindIII--2.14.
The sequence extends from position -98 to +4054 relative to the translation start site, and encodes a glycine at aa 185.
A full-length cDNA (coding sequence 3842 nt).
When inserted into a eukaryotic expression vector and transfected into BRO melanoma cells, the sequence confers multidrug resistance.
Constructed by adding XbaI linkers to the insert from an EcoRI-linkered library, and cloning into a derivative of pGEM-3Zf(-) in which the multiple cloning sequence had been replaced by a single XbaI site.
The insert is oriented with the 5' end closer to the T7 RNA polymerase promoter.
References
van der Bliek AM, et al. Sequence of mdr3 cDNA encoding a human P-glycoprotein. Gene 71: 401-411, 1988. PubMed: 2906314
Lincke CR, et al. Multidrug resistance phenotype of human BRO melanoma cells transfected with a wild-type human mdr1 complementary DNA. Cancer Res. 50: 1779-1785, 1990. PubMed: 1968359
