| 產品名稱 |
pGH205 |
| 商品貨號 |
B198156 |
| Designations |
pGH205 |
| Depositors |
OmniGene, Inc., S Guterman, OmniGene, Inc. |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Streptomyces lividans |
| Vector Information |
Size (kb): 12.1999998092651400 Vector: pGH205 (plasmid) Promoters: Promoter aph Construction: pGH202 pGH8 Marker(s):ampR tsrR Construct size (kb): 12.19999980926514 Features: marker(s): ampR
marker(s): tsrR
promoter: aph
replicon: pIJ101
replicon: pMB1
signal peptide coding region: endo H
coding sequence: endo H |
| Applications |
produces protein endo-beta-N-acetylglucosaminidase shuttle vector vector with sequences for protein export endo-beta-N-acetylglucosaminidase |
| Comments |
Shuttle expression vector permitting secretion of fusion proteins from S. lividans hosts. pGH202 (ATCC 39896) and pGH205 (ATCC 39895) differ in that the latter includes a promoter element for aminoglycoside phosphotransferase (aph). S. lividans transformed with this plasmid will secrete endo H when grown in this medium: Difco yeast extract (3 g/L), Difco peptone (5 g/L), Oxoid malt extract (3 g/L), glucose (10 g/L), sucrose (340 g/L), and 5 mM MgCl2. |
| Media |
YEME-thiostrepton medium
|
| Growth Conditions |
Temperature: 30.0°C |
| References |
Guterman S. Streptomyces secretion vector. US Patent 4,745,056 dated May 17 1988
Robbins PW, et al. Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosamine. J. Biol. Chem. 259: 7577-7583, 1984. PubMed: 6429133
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
