| 產品名稱 |
pAP12BD [pap12BD] |
| 商品貨號 |
B198172 |
| Designations |
pAP12BD [pap12BD] |
| Depositors |
Amgen, Inc., BJ Ratzkin, Amgen, Inc. |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli JA221 |
| Vector Information |
Size (kb): 15.0000000000000000 Vector: pAP12BD (plasmid) Promoters: Promoter PGK Construction: pBR322, Tn5 Marker(s):G418R,LEU2,ampR Construct size (kb): 15.0 Features: marker(s): ampR, LEU2, G418R
promoter: PGK
replicon: pMB1, 2 micron
terminator: PGK |
| Applications |
YE-type (episomal) shuttle vector shuttle vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--15.0; BglII--15.0; XhoI--9.4, 6.0; EcoRI--13.0, 2.1; KpnI--13.0, 2.2. Saccharomyces cerevisiae containing a single copy of the neomycin phosphotransferase gene are sensitive to G418; resistance to 400 ug/ml is expressed if there are several copies per cell. The promoter and terminator sequences were derived from the phosphoglycerate kinase gene. The G418-resistance segment includes a 0.7 kb region of the PGK promoter and a 1.5 kb BglII/BamHI fragment from Tn5. The BamHI site is between the G418R and the PGK terminator. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Patel AC, Ratzkin BJ. Materials and methods for controlling plasmid copy number and stability. US Patent 4,935,350 dated Jun 19 1990
|
| Shipped |
frozen |
| Shipping Information |
Distributed: freeze-dried |
