| 產品名稱 |
HFBEM33 |
| 商品貨號 |
B198389 |
| Designations |
HFBEM33 |
| GenBank Number |
T07654
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 7.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PTMA
Other gene symbols: TMSA
Gene name: prothymosin, alpha (gene sequence 28)
Contains complete coding sequence?: U
Localization: 2
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 4.4
Cross references: DNA Seq. Acc.: M14630; EST: EST05544
Nucleotides 9-285 of the insert correspond to
nucleotides 1200-924 of M14630 with 98% identity.
DNA Seq. Acc.: T07654; EST: EST05544; Primer: M13-21; THC
id: THC123467 Insert lengths(kb): 4.400000095367432 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: prothymosin, alpha (gene sequence 28)(prothymosin, alpha) [PTMA] |
| Insert Size (kb) |
4.400 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 3.1, 0.82, 0.32. The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs. Based on the alignment with GenBank M14630 (1200 nt, CDS 178-513) and the insert size, this clone probably contains the complete coding region. |
| References |
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
|
