| Vector |
Construct size (kb): 4.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: OGMT*
Gene name: 2-oxoglutarate carrier protein
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17, 23.7899999 cR
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: T08886; EST: EST06778; Primer: M13-21; THC
id: THC122477
DNA Seq. Acc.: T08887; EST: EST06779; Primer: M13 Reverse
DNA Seq. Acc.: X66114; EST: EST06779
Nucleotides 1...307 of the insert correspond to
nucleotides 45...864 of X66114.
DNA Seq. Acc.: X66114; EST: EST06778
Nucleotides 12-255, 256-318 of the insert correspond to
nucleotides 2518-2212 of X66114 with 95% identity. Insert lengths(kb): 1.5 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: 2-oxoglutarate carrier protein [OGMT*] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.75; HindIII/NotI--3.3, 1.5. Based on the alignment with GenBank accession X66114 (genomic sequence, 2518 nt), this clone appears to contain the complete coding sequence. |
