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pDP1125

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編號:B199101

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產(chǎn)品名稱	pDP1125
商品貨號	B199101
Designations	pDP1125
GenBank Number	M26946
Species	Homo sapiens, human
Depositors	DC Page
Vector	Construct size (kb): 6.0
Insert	DNA: cDNA Insert lengths(kb): 3.0 Tissue: male lymphoblastoid cell line WHT1659 Gene product: zinc finger protein, X-linked [ZFX] Alleles: A1, A2, A1, A2
Insert Size (kb)	3.0
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--3.0 (doublet); HindIII--6.2; BamHI--4.3, 2.1; SmaI--6.2; PstI--3.3, 1.1, 0.9, 0.88. The GenBank accession M26946 contains the sequence from 1228 to 2443 of the sequence in the Nature paper. pDP1125 is the 5' EcoRI fragment of cDNA2. It extends from nucleotide -426 through nucleotide +2592 of the published sequence. It includes the complete coding sequence with 5' and 3' flanking regions but does not include the Alu repeat. The clone detects transcripts of 6.3 and 8.0 kb from ZFX. It encodes the protein of 804 amino acids. The insert is oriented in the vector such that transcription from the T7 promoter gives the sense strand.
References	Schneider-Gadicke A, et al. ZFX has a gene structure similar to ZFY, the putative sex determinant, and excapes X inactivation. Cell 57: 1247-1258, 1989. PubMed: 2500252 Page DC, et al. Chromosomal localization of ZFX - a human gene that escapes X inactivation - and its murine homologs. Genomics 7: 37-46, 1990. PubMed: 1970799 Schneider-Gadicke A, et al. Putative transcription activator with alternative isoforms encoded by human ZFX gene. Nature 342: 708-711, 1989. PubMed: 2512506 David C Page, personal communication

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