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p79-2-23

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編號:B199814

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產(chǎn)品名稱	p79-2-23
商品貨號	B199814
Designations	p79-2-23
Species	Homo sapiens, human
Depositors	M Litt
Applications	Lathrop et al (HGM9) have used CEPH families to assign the following linkage group: cen- HP- CTRB- D16S20- pLC9- (APRT, D16S7)- qter.
Vector	Construct size (kb): 4.5
Insert	DNA: genomic Insert lengths(kb): 1.450000047683716 Gene product: DNA Segment, single copy [D16S7]
Insert Size (kb)	1.450
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.1, 1.5; PstI--3.2, 0.86; EcoRI--4.5; HindIII--4.5; SmaI--4.5. Lathrop et al (HGM9) have used CEPH families to assign the following linkage group: cen- HP- CTRB- D16S20- pLC9- (APRT, D16S7)- qter. Derived from cosmid CF33-79. The number of alleles detected is a function of population size and gel resolution; in a study of >1000 unrelated individuals another lab claims to have detected over 100 alleles. Use standard hybridization and washing conditions, with a final wash in 0.1X SSC/0.1% SDS at 65C.
References	Bufton L, et al. A highly polymorphic locus on chromosome 16q revealed by a probe from a chromosome-specific cosmid library. Hum. Genet. 74: 425-431, 1986. PubMed: 2878870 Chimera JA, et al. Population genetics of the highly polymorphic locus D16S7 and its use in paternity evaluation. Am. J. Hum. Genet. 45: 926-931, 1989. PubMed: 2574004 Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986 Michael Litt, personal communication

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