Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 0.3; EcoRI--4.9; SmaI--4.9; PstI--3.4, 1.15, 0.35. Acentric chromosome fragmentation vector targeting Alu sequences. Contains promoters for in vitro RNA synthesis. Any unique restriction site between the targeting sequence and the telomere-adjacent sequence can be used to linearize the plasmid before transformation. The EcoRV and ClaI sites between the SP6 promoter and the telomere sequence can be used for recovery from deletion derivatives of YAC insert sequences adjacent to the fragmentation site. Constructed from pBP103 (ATCC 77087) by inserting a 0.3 kb Alu-containing sequence from BLUR8 into the BamHI site. pBP108 (ATCC 77088) and pBP109 (ATCC 77089) differ in the orientation of the Alu sequence. The order of the major features in this plasmid is: bla - HIS3 - lacZ'/MCS/5'Alu3' - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI - EcoRV - SP6 promoter. |
