Rhizobium radiobacter (Beijerinck and van Delden) Young et al.
商品貨號(hào)
B201055
Deposited As
Agrobacterium tumefaciens
Strain Designations
NTL4(pZLR4)
Application
Bioreporter for detecting N-acyl homoserine lactone quorum-sensing signals.
Isolation
This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®
no
Type Strain
no
Comments
The depositor recommends using media supplemented with 25 mcg/ml gentamicin to select for plasmid retention.
Pzlr4 confers resistance to beta lactam antibiotics and gentamicin.
The plasmid also confers acyl-homoserine lactone-inducible production of β-galactosidase (LacZ) activity.
Medium
ATCC® Medium 1885: LB medium (ATCC medium 1065) with 15 mcg/ml gentamycin
Growth Conditions
Temperature: 28°C
Atmosphere: Aerobic
Name of Depositor
S Farrand
References
Cha C, et al. Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria. Mol. Plant Microbe Interact. 11(11): 1119-1129, 1998. PubMed: 9805399
