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Construct size (kb): 5.670000076293945

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSPORT1
Intact vector size: 4.109
Type of vector: plasmid
Vector end: NotI
Vector end: SalI
Cloning sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI
SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI
Sse8387I PstI
Polylinker sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI
SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI
Sse8387I PstI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: SP6, ->, 164-180
MCS: AatII...PstI, ->, 191-285
promoter for in vitro transcription: T7, <-, 298-318
insert detection: lacZ', <-, 1-341
repressor gene: lacI, <-, 464-1546
replicon: pMB1, 1903-1903
marker(s): ampR, <-, 2661-3521
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KIR
Other gene symbols: NKB1
Gene name: killer cell inhibitory receptor
ORF's seq. position: GDB probe: GDB:698358 ()
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.4; 19, 374.860000 cR
Tissue: Resting CD3- cells
Type of DNA: cDNA
Insert 5' end: Modification: SalI linker
Insert 3' end: Modification: NotI linker
Insert size (kb): 1.566
Cross references: DNA Seq. Acc.: U24077
Nucleotides 1-1532 of the insert correspond to
nucleotides 1-1532 of U24077.

Insert lengths(kb): 1.565999984741211

Tissue: Resting CD3- cells

Gene product: killer cell inhibitory receptor [KIR]

Restriction digests of the clone give the following sizes (kb): NotI/SalI--4.4, 1.6; EcoRV--5.7; PstI--5.0, 0.4, 0.35, 0.1; PvuII--3.8, 1.4, 0.6, 0.1; XhoI--5.7.

The insert contains the following restriction sites (approximate kb from the 5' end): BclI--0.35; PstI--0.04, 0.43, 0.51, 0.84; PvuII--1.12; SacI--0.45; StuI--1.05; XhoI--0.62.

Encodes a variant not recognized by either monoclonal antibody GL183 or EB6.