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Naegleria sp.

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編號:B202955

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產品名稱	Naegleria sp.
商品貨號	B202955
Strain Designations	SWL NG-005
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	freshwater, Wabash River, Attica, IN, 1964
Product Format	frozen
Type Strain	no
Comments	Zymodeme Gr-8 Equals NG-005, maximum temperature tolerance 40C This strain is a member of Naegleria sp. genetic cluster K. Genetic cluster K [Int. J. Parasitol. 19: 823-834, 1989] = Naegleria gruberi cluster 1 [J. Eukaryot. Microbiol. 40: 179-187, 1993].
Medium	ATCC^® Medium 997: Fresh water ameba medium
Growth Conditions	Max Temperature: 30.0°C Min Temperature: 25.0°C
Cryopreservation	1. ? Allow the cells to encyst.? To detach cysts from the plate flush the surface with 5 ml fresh ATCC medium 1323 (Page's Balanced Salt Solution).? Rub the surface of the plate with a spread bar to detach adhering amoebae. 2.?? Transfer the cyst suspension to a sterile centrifuge tube. 3. ? If the cyst concentration does not exceed 2 x 10⁶ cysts/ml adjust the suspension to that concentration.? To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 10⁶. 4.?? While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows:? Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath.? Allow the DMSO to solidify.? Add the required volume of refrigerated medium.? Dissolve the DMSO by inverting the tube several times.?
Name of Depositor	BS Robinson
Year of Origin	1964
References	De Jonckheere JF. A Group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification. J. Eukaryot. Microbiol. 40: 179-187, 1993. PubMed: 8461891 Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360 De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.

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