Chlamydophila pneumoniae (Grayston et al.) Everett et al.
商品貨號
B205001
Classification
Chlamydiaceae, Chlamydophila
Deposited As
Chlamydia pneumoniae Grayston et al.
Agent
Chlamydophila pneumoniae (Grayston et al.) Everett et al.
Strain
AO3
Applications
Respiratory research
Positive for Mycoplasma
Yes
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
First coronary isolate of C. pneumoniae.
FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies.
Recommended Host
HEp-2 cells (ATCC CCL-23)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, a 5% CO2 in air atmosphere is recommended
Name of Depositor
JT Summersgill
Special Collection
ATCC
Source
Coronary atheroma of human with athcroslerosis
References
Ramirez JA. Isolation of Chlamydia pneumoniae from the coronary artery of a patient with coronary atherosclerosis. The Chlamydia pneumoniae/Atherosclerosis Study Group. Ann. Intern. Med. 125: 979-982, 1996. PubMed: 8967709
This holding has tested positive for Mycoplasma contamination by PCR.
