| 產品名稱 |
Human rhinovirus 7 |
| 商品貨號 |
B205822 |
| Classification |
Picornaviridae, Rhinovirus, Human rhinovirus A |
| Agent |
Human rhinovirus 7 |
| Strain |
68-CV11 |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
?-70°C or colder |
| Comments |
Optimal conditions for grwoth include pH 8.6-7.3 in roller or rocker cultures incubated at approximately 33°C. All members of the rhinovirus group are ether resistant. |
| Effect on Host |
?CPE in culture include cell rounding, clumping, and sloughing. |
| Recommended Host |
Production host - HeLa cells (ATCC CCL-2)
Alternate host(s) - HD, HEK, MRC-5, MkK, WI-38 Recommended Host: ??HeLa cells (ATCC CCL-2?) |
| Growth Conditions |
For best results, plant cells 24-48 hours prior to infection. Infect at 70-80% confluence using a multiplicity of infection (MOI) of 0.1-1. Incubate 1-7 days at 33°C, rocking, in a humidified 5% CO2 atmosphere. Harvest culture fluids or culture fluids plus cells. Freeze as rapidly as possible. |
| Name of Depositor |
NIAID, ATCC |
| Source |
Derived from NIAID reagent V-127-001-021 (VR-117) by passage at ATCC |
| References |
Hamparian VV, et al. Recovery of new viruses (coryzavirus) from cases of common cold in human adults. Proc. Soc. Exp. Biol. Med. 108: 444-453, 1961. PubMed: 13904225
NIAID Catalog of Research Reagents 1978-1980. S. Cunningham, ed. US Department of Health Education and Welfare, Public Health Service, National Institutes of Health; DHEW Pulication No. (NIH) 78-899, Bethesda, MD.
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