| Vector Information |
Intact vector size: 5.437
Type of vector: phagemid
Cloning sites: NheI EcoRI SacI KpnI XbaI SalI PstI SphI
Polylinker sites: NheI EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI PstI SphI
HindIII
Construction: pBAD18, kanR (pUC4K)
Host range: Escherichia coli
Features (with orientation and position when available):
regulator: araC, ←, 3346-4224
operator: O2, 4253-4270
promoter: araC, ←, 4375-4403
operator: O1, 4411-4432
other: CAP site, 4454-4467
operator: I2 + I1, 4463-4501
promoter for expression: arabinose BAD, →, 4500-4527
MCS: NheI...HindIII, ->, 4550-4612
transcription terminator: rrnB T1 + T2, →, 4613-5038
marker(s): kanR, →, 123-938
replicon: M13, 1417-1875
replicon: pMB1, 1881-2578 |
| References |
Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087
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