Restriction digests of the clone give the following sizes (kb): NotI--5.5, 1.5; BamHI--4.6, 2.3; BglI--3.6; 1.9, 1.1, 0.4; PvuII--4.8, 2.1; ClaI--4.2, 2.35, 0.5. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.42; BglI--0.87, 1.25; ClaI--0.83; PstI--0.14, 0.29, 0.71; PvuII--1.06; SphI--1.3. Contains the complete coding sequence (nt 1-1191). Can complement ura1 mutants in Saccharomyces cerevisiae. Detects a unique 1.6 kb transcript in humans. |
