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pDP34

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編號:B210648

品牌:Mingzhoubio

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產(chǎn)品名稱	pDP34
商品貨號	B210648
Designations	pDP34
Depositors	DC Page
Vector	Construct size (kb): 6.599999904632568
Insert	DNA: genomic Insert lengths(kb): 2.200000047683716 Gene product: DNA Segment, single copy, homologous on X and Y [DXYS1] Alleles: B1, B2, A1, A2, B1, B2, A1, A2
Insert Size (kb)	2.200
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--2.25 kb triplet; HindIII--2.4 kb, 2.2 kb doublet; BamHI--6.6; PstI--2.2, 1.6, 1.5, 1.3. As there is only 1 BamHI site, there seem to be 2 inserts. pDP34 corresponds to probe 7 in Fig. 2. pDP34 shows linkage at a LOD score of 3.07 (theta=0) to cleft palate + ankyloglossia in a large Icelandic family. DXYS1 is linked to choroideremia with theta max = 0.02, LOD score = 10.25. This segment is deleted in XL-62 family. The insert detects a TaqI RFLP which maps to Xq21. The insert detects a MspI RFLP which maps to Yp. To avoid lane background, hybridize at high stringency (e.g., 47C in 50% formamide, 5X SSC; wash at 60C in 0.1X SSC, 0.1% SDS).
References	Page DC, et al. Occurrence of a transposition from the X-chromosome long arm to the Y-chromosome short arm during human evolution. Nature 311: 119-123, 1984. PubMed: 6088994 Page D, et al. Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes. Proc. Natl. Acad. Sci. USA 79: 5352-5356, 1982. PubMed: 6291041 Merry DE, et al. Choroideremia and deafness with stapes fixation: a contiguous gene deletion syndrome in Xq21. Am. J. Hum. Genet. 45: 530-540, 1989. PubMed: 2491012 Schwartz CE, et al. Allan-Herndon syndrome. II. Linkage to DNA markers in Xq21. Am. J. Hum. Genet. 47: 454-458, 1990. PubMed: 2393020 Moore GE, et al. Linkage of an X-chromosome cleft palate gene. Nature 326: 91-92, 1987. PubMed: 2881212 Lesko JG, et al. Multipoint linkage analysis of loci in the proximal long arm of the human X chromosome: Application to mapping the choroideremia locus. Am. J. Hum. Genet. 40: 303-311, 1987. PubMed: 2883887 David C Page, personal communication

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