天天操夜夜拍丨国产精品超清白人精品av丨天天爽天天噜在线播放丨国产精品久久久久久久福利竹菊丨色综合天天天天做夜夜夜夜做丨国内自拍xxxx18丨白人と日本人の交わりビデオ丨午夜激情在线观看丨成人18视频丨久久66热人妻偷产精品丨视频一区二区三区在线观看丨操操操插插插丨久久的久久爽亚洲精品aⅴ丨91精品一区二区三区在线观看丨aaa女人18毛片水真多丨99re在线视频精品丨超碰免费在丨中文字幕人妻无码专区app丨午夜香蕉视频丨又大又粗欧美黑人aaaaa片丨久久夫妻视频丨乌克兰性欧美精品高清丨亚洲欧美福利视频丨少妇高潮久久久久久一代女皇丨97国产高清

Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 5.6999998092651370

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: cosmid
Vector end: NdeI
Vector end: NdeI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752

Vector: pBG1 (plasmid)

Promoters: Promoter lac

Construction: pUC18, ars1, his3

Marker(s):ampR,his3

Construct size (kb): 5.699999809265137

Features: insert detection: lacZ'
marker(s): ampR
marker(s): his3+
promoter: lac
replicon: ars1
replicon: pMB1
MCS: SacI...SphI

contains sequence autonomous replication sequence

contains sequence imidazole acetol phosphate transaminase

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.2, 0.55; DraI/EcoRI--2.5, 2.4, 0.7; HindIII--3.5, 2.2; PstI--5.7.

The his3 insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.49; ClaI--0.04; EcoRI--0.47, 1.01; HindIII--1.03; PvuI--0.04; XbaI--0.27; XhoI--0.84.

Temperature: 37.0°C

component of:ATCC 87686