| Growth Conditions |
Temperature: 35°C to 38°C
Incubation: 1 to 4 days at 37°C, rocking at setting #1, a 5%CO2 in air atmosphere is recommended.
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent). |
| References |
Acree WM, et al. Adjuvants for vaccines. US Patent 5,047,238 dated Sep 10 1991
Acree WM, et al. Inactivated canine coronavirus vaccine. US Patent 4,567,042 dated Jan 28 1986
Acree WM, et al. Canine corona virus vaccine. US Patent 4,567,043 dated Jan 28 1986
Acree WM, et al. Canine corona virus vaccine. US Patent 4,824,785 dated Apr 25 1989
Acree WM, Black JM. Canine corona virus vaccine. US Patent 5,013,663 dated May 7 1991
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