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pHDR5A

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編號:B212702

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產品名稱	pHDR5A
商品貨號	B212702
Designations	pHDR5A
GenBank Number	M14758
Species	Homo sapiens, human
Depositors	MM Gottesman
Vector	Construct size (kb): 4.199999809265137
Insert	DNA: cDNA Insert lengths(kb): 1.379999995231628 Tissue: carcinoma KB-C2.5 cell line Gene product: P glycoprotein 1/multiple drug resistance 1 [MDR1]
Insert Size (kb)	1.380
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	The insert contains the following restriction sites (at nucleotide positions according to the published sequence): PvuII--1314, XmnI--1560, PvuII--1921, HindIII--2041; StuI--2168; XmnI--2292. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; HindIII--3.3, 0.9; BamHI--4.3; SacI--4.3. The insert includes nucleotides 1178 to 2561 of the cDNA. This probe detects a mRNA of approximately 4.4-4.5 kb. The insert is oriented such that transcription from the T7 promoter gives the sense strand.
References	Galski H, et al. Expression of a human multidrug resistance cDNA (MDR1) in the bone marrow of transgenic mice: resistance to daunomycin-induced leukopenia. Mol. Cell. Biol. 9: 4357-4363, 1989. PubMed: 2573831 Chen CJ, et al. Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell 47: 381-389, 1986. PubMed: 2876781 Ueda K, et al. The human multidrug resistance (mdr1) gene: cDNA cloning and transcription initiation. J. Biol. Chem. 262: 505-508, 1987. PubMed: 3027054 Michael M Gottesman, personal communication

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