| 產品名稱 |
RATAOGEN |
| 商品貨號 |
B213096 |
| Designations |
RATAOGEN |
| GenBank Number |
M12112
M12113
|
| Species |
Rattus norvegicus, rat |
| Depositors |
KR Lynch |
| Vector |
Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4Z
Intact vector size: 3.000
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: AGT
Genomic copy number: unique
Gene name: angiotensinogen
Contains complete coding sequence?: Y
Strain: Sprague-Dawley
Tissue: kidney, male
Type of DNA: cDNA
Insert end: EcoRI linker Modification: blunted
Insert end: EcoRI linker Modification: blunted
Insert size (kb): 1.6
Cross references: DNA Seq. Acc.: M12112
DNA Seq. Acc.: M12113 Insert lengths(kb): 1.600000023841858 Tissue: kidney, male Gene product: angiotensinogen(angiotensin) [AGT] |
| Insert Size (kb) |
1.600 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 1.6; BamHI--3.0, 0.75, 0.5; HindIII--4.3. |
| References |
Lynch KR, et al. Localization of preangiotensinogen messenger RNA sequences in the rat brain. Hypertension 8: 540-543, 1986. PubMed: 3710560
|
