| Insert |
DNA: cDNA Insert lengths(kb): 4.5 Tissue: colon carcinoma T84 cell line Gene product: cystic fibrosis transmembrane conductance regulator [CF] Alleles: A1, A1, A1, A1, A2, B1, B2, A1, A1, A2, B1, B2, A2, A2, A2, A2 |
| Comments |
This insert contains 2 internal EcoRI sites. An EcoRI digest results in fragments of 1.2, 1.5, and 1.8 kb from the insert. Restriction digests of the clone give the following sizes (kb): EcoRI--2.85, 1.85, 1.5, 1.35; XbaI--7.7; PstI--7.7; HincII--3.65, 1.0, 0.92, 0.77, 0.68, 0.54, 0.43; HindIII--3.45, 2.0, 1.0, 0.92. ATCC 61151 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not needed for ATCC 61151, but is for ATCC 61150. This probe contains an extraneous sequence between exons 10 and 11. This clone includes exons 7 - 24 and hybridizes to genomic EcoRI fragments of 9.3, 10.3, 4.3, 3.5, 2.4, 0.5, 1.8, 1.8, 3.5, 1.9, 4.5, 2.4, 1.0, 2.8, 1.8, 8.5, 2.2, and 2.3 kb. |
