| 產品名稱 |
pBC-KC |
| 商品貨號 |
B213669 |
| Designations |
pBC-KC |
| GenBank Number |
J04596
|
| Species |
Mus musculus, mouse |
| Depositors |
CD Stiles |
| Applications |
It is frequently used as a molecular marker for response to cellular growth factors. |
| Vector |
Construct size (kb): 3.799999952316284 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.800000011920929 Tissue: 3T3 (clone A31) cell line Gene product: GRO1 oncogene (melanoma growth stimulating activity, alpha)(growth regulated (alpha), growth regulated (group I)) [Mgsa] |
| Insert Size (kb) |
0.800 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--2.8, 0.84; EcoRI--3.85; HindIII--3.85. KC is one of several competence genes (including JB, JE) that are induced with the addition of platelet-derived growth factor. It is frequently used as a molecular marker for response to cellular growth factors. Detects a mRNA of 1.2 kb after PDGF induction. The level of induction is increased by cycloheximide treatment. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Cochran BH, et al. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell 33: 939-947, 1983. PubMed: 6872001
Sakaguchi AY, et al. Mouse melanoma growth stimulator activity gene (Mgsa) is polymorphic and syntenic with the W, patch, rumpwhite, and recessive spotting loci on chromosome 5. Genomics 5: 629-632, 1989. PubMed: 2575589
Oquendo P, et al. The platelet-derived growth factor-inducible KC gene encodes a secretory protein related to platelet alpha-granule proteins. J. Biol. Chem. 264: 4133-4137, 1989. PubMed: 2917992
|
