Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Human clinical specimen, Fajara Gambia, 1976
Product Format
frozen
Storage Conditions
Frozen: -70°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Protocols Section
Type Strain
no
Medium
ATCC® Medium 2196: Malaria medium, complete
Growth Conditions
Temperature: 37°C
Culture System:In vitro culture in human erythrocytes
Cryopreservation
Reagents Glycerolyte medium**
Glycerol: 57.00 g
NaC3H5O3 (Sodium lactate): 1.60 g
KCl: 30.00 mg
NaH2PO4: 1.38 g
Glass distilled water: 100.00 mL
Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave and store at 4°C.
Harvesting and Preservation
Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.
Centrifuge ring-stage culture for 5 mins at 1000 x g in 50 mL centrifuge tube.
Aspirate supernatant using sterile Pasteur pipet.
Resuspend pellet gently in remaining supernatant.
Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
Add the remaining 4 volumes of glycerolyte and gently agitate.
Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.).
Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor
W Trager
Special Collection
ATCC
Year of Origin
1976
References
Isolated from an infected human blood sample collected by M. Friedman, Medical Research Council Labs Clinic, Fajara, Gambia, 1976.
Jensen JB, et al. Clinical drug-resistant falciparum malaria acquired from cultured parasites. Am. J. Trop. Med. Hyg. 30: 523-525, 1981. PubMed: 7020443
Jensen JB, Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am. J. Trop. Med. Hyg. 27: 743-746, 1978. PubMed: 356635
Jensen JB, Trager W. Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method. J. Parasitol. 63: 883-886, 1977. PubMed: 335035
