| 產品名稱 |
BLUR8 [pPD8] |
| 商品貨號 |
B217067 |
| Designations |
BLUR8 [pPD8] |
| GenBank Number |
J00091
|
| Species |
Homo sapiens, human |
| Depositors |
PL Deininger |
| Vector |
Construct size (kb): 4.639999866485596 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Genomic copy number: repetitive
Gene name: Alu consensus sequence, repetitive
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 0.275
Cross references: DNA Seq. Acc.: J00091 Insert lengths(kb): 0.2750000059604645 Gene product: Alu consensus sequence, repetitive |
| Insert Size (kb) |
0.275 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
EcoRI--4.7; HindIII--4.7; BamHI--4.4, 0.27; PvuII--4.7; PstI--4.7. Because of the BamHI linkers (CCGGATCCGG), the size of the excised fragment is 275 bp, with 265 bp representing the Alu repeat. Consists of a head to tail dimer of approximately 130 bp with an insert of 31 bp in the right half as compared to the left. There is a 22% mismatch between BLUR8 and the average Alu family sequence (cf. GenBank accession J00092). |
| References |
Rubin CM, et al. Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences. Nature 284: 372-374, 1980. PubMed: 6244506
Deininger PL, et al. Base sequence studies of 300 nucleotide renatured repeated human DNA clones. J. Mol. Biol. 151: 17-33, 1981. PubMed: 6276559
Prescott L Deininger, personal communication
|
