Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
prehensile-tailed porcupine, Coendou rothschildi, Sta Rita, Chorrera, Panama, 1972
Product Format
frozen
Type Strain
no
Comments
Promastigotes.
species description
lipids
Cyclopropane fatty acid
Medium
ATCC® Medium 811: Leishmania medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor
NR Dollahon
Chain of Custody
ATCC <<--NR Dollahon<<--A. Herrer
Year of Origin
1972
References
Herrer A. Leishmania hertigi sp. n., from the tropical porcupine, Coendou rothschildi Thomas. J. Parasitol. 57: 626-629, 1971. PubMed: 5090970
Beach DH, et al. Lipids of Leishmania promastigotes. J. Parasitol. 65: 201-216, 1979. PubMed: 448607
Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247
