| Vector Information |
Size (kb): 5.0710000991821290 Vector: pKSM710 (phagemid) Promoters: Promoter for expression lambda PL Construction: pSM702, pMAMPF3 Marker(s):ampR,cmlR (am) Construct size (kb): 5.071000099182129 Features: insert detection: lacZalpha
marker(s): ampR
marker(s): cmlR (am)
promoter for expression: lacUV5
promoter for expression: lambda PL
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--4.5, 0.5; PstI--5.0, DraIII--3.0, 2.1; DraIII/PvuI--2.0, 1.6, 0.9, 0.6. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--4.43; BglI--1.87; BglII--4.09; EcoRI--4.03, 4.48; NcoI--4.42; PstI--2.00; PvuI--2.13, 4.60; PvuII--3.40, 4.57. Expression vector containing a 2.57 kb BglII-BspHI cassette useful for constructing a variety of expression vectors. |
