| 產品名稱 |
HFBCX08 |
| 商品貨號 |
B221061 |
| Designations |
HFBCX08 |
| GenBank Number |
M85990
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 5.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RAB5B
Genomic copy number: unique
Gene name: RAB5B, member RAS oncogene family
Contains complete coding sequence?: Y
Chromosome: 12; Localization: 12 q13
Genome: Homo sapiens
Gene symbol: RAB5B
Genomic copy number: unique
Gene name: guanine nucleotide-binding protein Rab5B, ras-oncogene
related
Contains complete coding sequence?: Y
Chromosome: 12; Localization: 12 q13
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.4
Cross references: DNA Seq. Acc.: M85990; EST: EST02515; Primer: M13 Forward;
THC id: THC209023
DNA Seq. Acc.: T05431; EST: EST03320; Primer: M13 Reverse;
THC id: THC209023
DNA Seq. Acc.: X54871; EST: EST02515; THC id: THC209023
Nucleotides 6-291 of the insert correspond to
nucleotides 1-284 of X54871 with 99% identity. Insert lengths(kb): 2.400000095367432 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: RAB5B, member RAS oncogene family( guanine nucleotide-binding protein Rab5B, ras-oncogene related, GTP-binding protein rab5B) [RAB5B] |
| Insert Size (kb) |
2.400 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.0, 2.4. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.4; BamHI--5.4; PvuII--2.6, 1.7, 0.7, 0.5. |
| References |
Korenberg JR, et al. Toward a cDNA map of the human genome. Genomics 29: 364-370, 1995. PubMed: 8666383
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
|
