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pIP82

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編號:B222499

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產品名稱	pIP82
商品貨號	B222499
Designations	pIP82
GenBank Number	X64810
Species	Homo sapiens, human
Applications	produces protein proprotein convertase subtilisin/kexin type 1 neuroendocrine convertase 1 (pro-protein-convertase PC1)
Vector	Construct size (kb): 6.099999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-3Zf(+) Intact vector size: 3.199 Type of vector: phagemid Vector end: XbaI Vector end: XbaI Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII-SP6 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ' marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PCSK1 Other gene symbols: NEC1 Genomic copy number: unique Gene name: proprotein convertase subtilisin/kexin type 1 Contains complete coding sequence?: Y Chromosome: 5; Localization: 5 q15-q21 Tissue: lung Type of DNA: cDNA Insert end: XbaI Insert end: XbaI Insert size (kb): 2.9 Cross references: DNA Seq. Acc.: X64810 Insert lengths(kb): 2.900000095367432 Tissue: lung Gene product: proprotein convertase subtilisin/kexin type 1(neuroendocrine convertase 1 (pro-protein-convertase PC1)) [NEC1] Target Gene: proprotein convertase subtilisin/kexin type 1
Insert Size (kb)	2.900
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments	Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--3.2, 2.9; BamHI--4.1, 2.0; EcoRI--3.5, 1.9, 0.65; PstI--3.3, 2.8; SacI--3.6, 2.6. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.12; EcoRI--1.82; BamHI--1.97. The 3' XbaI site is not cleaved by XbaI. Sequence analysis indicates that the site is present. Corresponds to nt 87-3039 of the sequence record. Contains the complete coding sequence (nt 190-2451 of the sequence record).
References	Creemers JW, et al. Expression in human lung tumor cells of the proprotein processing enzyme PC1/PC3. Cloning and primary sequence of a 5 kb cDNA. FEBS Lett. 300: 82-88, 1992. PubMed: 1547893 Wim J Van De Ven, personal communication

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