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lambda92 [GC.92]

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編號(hào):B223631

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產(chǎn)品名稱	lambda92 [GC.92]
商品貨號(hào)	B223631
Designations	lambda92 [GC.92]
GenBank Number	L10641
Species	Homo sapiens, human
Depositors	A Dugaiczyk
Vector	Construct size (kb): 48.79999923706055 DESCRIPTION OF VECTOR COMPONENT: Name of vector: Charon 4A Intact vector size: 46.000 Type of vector: phage Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none promoter: none replicon: lambda terminator: none Cross references:
Insert	DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GC Genomic copy number: unique Gene name: group-specific component (vitamin D binding protein) Contains complete coding sequence?: N Chromosome: 4; Localization: 4 q12-q13 Tissue: fetal liver (ATCC 37333) Type of DNA: genomic Insert end: HaeIII or AluI Modification: EcoRI linker Insert end: HaeIII or AluI Modification: EcoRI linker Insert size (kb): 17.85 Cross references: DNA Seq. Acc.: L10641 Nucleotides ~1-17850 of the insert correspond to nucleotides ~19800-37650 of L10641. Insert lengths(kb): 17.85000038146973 Tissue: fetal liver (ATCC 37333) Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]
Insert Size (kb)	17.850
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried bacteria-free lysate
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 6.2, 3.5, 2.7, 2.5, 1.9, 1.15; HindIII--18 (doublet), 6.2, 2.4, 1.4, 1.1, 1, 0.4; BamHI--14 (doublet), 8, 6.2, 4, 1.5, 0.5; SstI-->23, 8.5, 4.2; BglII--18, 5.2, 4.85, 4.7, 3.7, 2.2, 2.1, 1.9, 1.4, 1.0, 0.9, 0.8, 0.75, 0.65, 0.4, 0.3. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.9, 1.05, 2.95, 5.45, 11.65, 14.35; SacI--2.4; HindIII--8.1, 10.4, 11.5, 12.8, 13.8, 14.2. Begins in intron 2 and extends into intron 11. ATCC 95664/95665 and 95666/95667 overlap by 6.45 kb and share EcoRI fragments of the following sizes (kb): 0.15, 1.9, 2.5. ATCC 95666/95667 and 95668/95669 overlap by 7.8 kb and share an EcoRI fragment of 2.7 kb. Contains Alu repeats, Kpn repeats, and a THE repeat.
References	Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650

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