The insert contains the following restriction sites, in base pairs from the 5' end: BamHI--85; PstI--534; EcoRI--656; EcoRV--828. Restriction digests of the clone give the following sizes (kb): EcoRI--2.4, 0.85, 0.65; BamHI--2.5, 1.4; PstI--2.95, 0.95; HindIII--3.9. The insert includes 283 bp of 5' untranslated sequence and encodes the signal peptide and the amino terminal portion of the collagenous domain. Detects transcripts of 6.3 kb. |
