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Naegleria pringsheimi De Jonckheere

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編號:B242021

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產(chǎn)品名稱	Naegleria pringsheimi De Jonckheere
商品貨號	B242021
Deposited As	Naegleria gruberi Schardinger
Strain Designations	CCAP 1518/1c
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	dried
Type Strain	no
Comments	Zymodeme Gr-4 CCAP 15181/c is no longer available from CCAP Maximum temperature tolerance 37C Equivalent to CCAP 1518/1c, Group I intron in SSUrRNA gene biochemical identification genetic cluster M Genetic cluster M [RF34321] = Naegleria gruberi cluster 3 [RF34247].
Medium	ATCC^® Medium 997: Fresh water ameba medium
Growth Conditions	Temperature: 25.0°C Duration: grown with Escherichia coli ATCC 11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Subcultivation	Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Cryopreservation	1. ? Allow the cells to encyst.? To detach cysts from the plate flush the surface with 5 ml fresh ATCC medium 1323 (Page's Balanced Salt Solution).? Rub the surface of the plate with a spread bar to detach adhering amoebae. 2.?? Transfer the cyst suspension to a sterile centrifuge tube. 3. ? If the cyst concentration does not exceed 2 x 10⁶ cysts/ml adjust the suspension to that concentration.? To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 10⁶. 4.?? While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows:? Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath.? Allow the DMSO to solidify.? Add the required volume of refrigerated medium.? Dissolve the DMSO by inverting the tube several times.?
Name of Depositor	CCAP
Chain of Custody	ATCC <<--CCAP<<--E.G. Pringsheim
References	Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 Page FC. Morphological variation in the cyst wall of Naegleria gruberi (amoebida, vahlkampfiidae). Protistologica 11: 195-204, 1975. De Jonckheere JF. A Group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification. J. Eukaryot. Microbiol. 40: 179-187, 1993. PubMed: 8461891 Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360 De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002. name change from Naegleria gruberi to N. pringsheimi per TA Nerad, by e-mail message dated Dec 9 2002

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