天天操夜夜拍丨国产精品超清白人精品av丨天天爽天天噜在线播放丨国产精品久久久久久久福利竹菊丨色综合天天天天做夜夜夜夜做丨国内自拍xxxx18丨白人と日本人の交わりビデオ丨午夜激情在线观看丨成人18视频丨久久66热人妻偷产精品丨视频一区二区三区在线观看丨操操操插插插丨久久的久久爽亚洲精品aⅴ丨91精品一区二区三区在线观看丨aaa女人18毛片水真多丨99re在线视频精品丨超碰免费在丨中文字幕人妻无码专区app丨午夜香蕉视频丨又大又粗欧美黑人aaaaa片丨久久夫妻视频丨乌克兰性欧美精品高清丨亚洲欧美福利视频丨少妇高潮久久久久久一代女皇丨97国产高清

Distribution host: Escherichia coli MKH13 (ATCC 47087)

Size (kb): 5.3800001144409180

DESCRIPTION OF VECTOR:
Intact vector size: 5.380
Type of vector: plasmid
Cloning sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI
SacI SphI EcoRV NcoI HindIII
Polylinker sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI
SacI PstI SphI EcoRV NcoI StyI HindIII
Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR, ->, 84-1274
replicon: rop (copy number control), 1913-2104
replicon: pMB1, 2532-2532
marker(s): ampR, <-, 3291-4161
transcription terminator: rrnB T2, <-, 4263-4340
transcription terminator: rrnB T1, <-, 4422-4465
MCS: BamHI...HindIII, <-, 4749-4672
coding sequence: proV 5' sequence, <-, 4756-5065
promoter for expression: proU, <-, 5063-5366

Vector: pOSEX2 (plasmid)

Promoters: Promoter for expression proU

Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322

Marker(s):ampR,tetR

Construct size (kb): 5.380000114440918

Features: marker(s): ampR
marker(s): tetR
promoter for expression: proU
replicon: pMB1
replicon: rop (copy number control)
MCS: BamHI...HindIII
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence

expression vector

Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.05; HindIII--4.6, 0.8; PvuII--5.4.

Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride.

Vector does not provide a ribosome binding site or initiation codon.

Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter.

This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor.

Temperature: 37.0°C