| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--18.9, 7.4, 7.2, 5.2, 3.6, 1.4; EcoRI--30.7, 12.1, 4.4; HindIII--24.3, 9.9, 7.6, 4.4, 4.0; BglII--23.1, 12.5, 11.4, 4.7; SmaI--20.9, 8.9, 8.5, 6.9, 5.5. A 6.4 kb SmaI fragment of this construct (62 bp 5' untranslated, the coding sequence, through 2.0 kb of the right arm of lambdagt10) was inserted into pSAD and transfected into COS-7 cells. Immunoreactive membrane protein was detected. The insert includes nucleotides 328-4848 of the composite cDNA sequence, encompassing about 115 bp 5' untranslated, the complete coding sequence of 3621 bp, and 791 bp of 3' untranslated sequence. |
| References |
Bell GI, et al. Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization. Nucleic Acids Res. 14: 8427-8446, 1986. PubMed: 3491360
|
