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pUR278-183K
pUR278-183K
規格:
貨期:
編號:B243921
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pUR278-183K
商品貨號 B243921
Designations pUR278-183K
Species Tobacco mosaic virus
Depositors WO Dawson
Applications
produces protein 183K protein
Vector
Construct size (kb): 6.329999923706055
Insert
DNA: cDNA
Insert lengths(kb): 1.129999995231628
Gene product: 183K protein
Target Gene: 183K protein
Insert Size (kb) 1.130
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): PstI--5.6, 0.8; EcoRI--5.6, 0.8, 0.5; HindIII--6.8; BamHI/ClaI--3.3, 2.3, 1.25; BamHI--6.8; ClaI--3.6, 3.2.
This clone expresses an antigen specific for the 183K protein of tobacco mosaic virus.
A fragment containing the 183K gene (nt 3572 to approx. 4700) of pTMV204 was ligated into pUR278.
Classification Tobamovirus
References

Dawson WO, et al. cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc. Natl. Acad. Sci. USA 83: 1832-1836, 1986.

Lehto K, et al. Time course of TMV 30K protein accumulation in intact leaves. Virology 174: 290-293, 1990. PubMed: 2294644

William O Dawson, personal communication

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