Arthrobacter protophormiae (Lysenko) Stackebrandt et al.
Depositors
New England Biolabs, Inc.
Applications
in suitable host, produces protein modification methylase ApoI
in suitable host, produces protein restriction endonuclease ApoI
Vector
Construct size (kb): 9.2
Insert
DNA: genomic
Insert information:
Insert size (kb): 4.8
Gene : restriction endonuclease ApoI, modification methylase ApoI
DNA : genomic
Source : Arthrobacter protophormiae ATCC 55228
Insert ends : BglII
Insert lengths(kb): 4.80
Gene product: restriction endonuclease ApoI [hsdM]
Target Gene: modification methylase ApoI, restriction endonuclease ApoI
Insert Size (kb)
4.800
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.8, 2.5, 1.5, 0.5; HindIII--6.7, 2.5.
The insert contains the following restriction sites (approximate kb from one end): PstI--1.25, 1.75, 3.6; BamHI--1.83, 2.6, 2.8, 4.5; HindIII--2.08.
Contains and expresses the complete methylase and endonuclease genes.
Distributed in aliquots of 200 - 250 ng (20-25 ng/ul).
References
Polisson C, et al. Novel type II restriction endonuclease, ApoI, obtainable from Arthrobacter protophormiae and a process for producing the same. US Patent 5,200,337 dated Apr 6 1993
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
